TABLE 1.
Most commonly used lectins for the enrichment of glycoproteins and glycopeptides.
| Common Abbreviation | Source | Specificity | Competitive sugar or elution conditions/affinity-promoting metal ions | References |
|---|---|---|---|---|
| AAL | Aleuria aurantia | Fuc α1-6 [Core Fuc] > Fucα1-3, Fucα1-2 | Fuc/− | Matsumura et al. (2007) |
| AOL | Aspergillus oryzae | Fuc α1-6 [CoreFuc] > Fuc α1-2 > Fuc α1-3, Fuc α1-4 | Fuc/− | Matsumura et al. (2007) |
| Con A | Canavalia ensiformis (concanavalin A) | high-Man, hybrid type and complex biantennary N type | α-MM, α-MG/Mg2+, Mn2+, and Ca2+ | Ogata et al. (1975) |
| DSL, DSA | Datura stramonium | β-GlcNAc: 2,6-branched complex tetra/triantennary > 2,4-branched complex tetra/triantennary N type | acidic conditions *, − | Kawashima et al. (1990) |
| ECL, ECA | Erythrina Cristagalli | Lactosamine: Gal β 1-4GlcNAc | Lac/Ca2+, Mn2+, and Zn2+ | Wu et al. (2007) |
| GNL, GNA | Galanthus Nivalis | Core Man: monoantenary N type galacto&agalacto > bi/triantenary | α-MM/− | Shibuya et al. (1988) |
| HPA | Helix pomatia | T antigen | GlcNAc/− | Piller et al. (1990) |
| AIA | Artocarpus integrifolia (Jacalin) | Tn, T, sialyl T, and Core 3 | Gal or Mel/− | Tachibana et al. (2006) |
| LcH,LCA | Lens culinaris | mono/biantenary N-type with Core Fuc [Fuc α1-6] | α-MM, α-MG/Ca2+, and Mn2+ | Chandrasekaran et al. (2016) |
| LEL,TL | Lycopersicon esculentum | Polylactosamine: [(Gal β1-4GlcNAc)n, n ≥ 3], Lactosamine | acidic conditions/− | Kawashima et al. (1990) |
| LTL | Lotus tetragonolobus | Fuc α1-3/-2 (Gal β1-4)GlcNAc | Fuc/Ca2+, Mn2+ | Bhattacharyya et al. (1990) |
| MAL I | Maackia amurensis | sialyllactosamine: Sia α2-3Gal β1-4GlcNAc | Lac/− | Knibbs et al. (1991) |
| MAL II, MAH | Maackia amurensis | disialyl-T | acidic conditions/− | Konami et al. (1994) |
| PHA-E | Phaseolus vulgaris Erythroagglutinin | Bisecting GlcNAc in galactosylated complex tetra/triantennary N-type | acidic conditions/Ca2+, Mn2+ | Kaneda et al. (2002) |
| PHA-L | Phaseolus vulgaris Leucoagglutinin | Gal: 2,6-branched complex tetra/triantennary > complex bi antennary | acidic conditions, GlcNac/Ca2+, Mn2+ | Kaneda et al. (2002) |
| PNA | Arachis hypogaea (peanut aggluttinin) | T, disialyl-T > core 2 | Gal/Ca2+, Mg2+ | Chandrasekaran et al. (2016) |
| PSA | Pisum sativum | mono/bianntenary N-type with Core Fuc [Fuc α1-6] | α-MM, α-MG/Ca2+, Mn2+ | Tateno et al. (2009) |
| RCA I, RCA120 | Ricinus communis I | Gal β1-4GlcNAc > Gal β1-3GlcNAc > core 2 | Gal or Lac | Chandrasekaran et al. (2016) |
| SNA | Sambucus nigra | Sia α2-6Gal (NAc)-R | Lac, acidic conditions | Fischer and Brossmer, (1995) |
| UEA-I | Ulex europaeus | Fuc α1-2 [terminal] > Fuc α1-4 [subterminal] | Fuc/Ca2+, Mn2+, and Zn2+ | Allen et al. (1977) |
| VVL, VVA | Vicia villosa | Tn | GalNAc/Ca2+, Mn2+ | Puri et al. (1992) |
| WFA, WFL | Wisteria floribunda | GalNAc, GalNAc β1-4GlcNAc, Gal β1-3/-6Gal (NAc) | GalNAc, Lac/− | Piller et al. (1990) |
| WGA | Triticum vulgaris (weat germ agglutinin) | Chitin, GlcNAcβ 1-4GlcNAc, Sia (multivalent) | GlcNAc/Ca2+ | Gallagher et al. (1985), Chandrasekaran et al. (2016) |
Notes: α-MM: methyl α-D-mannopyranoside; α-MG: methyl α-D-glucopyranoside; Ca: calcium; Chitin: GlcNAc(β1−4)n; Core 2: GlcNAcβ1-6(Galβ1-3)GalNAc; Core 3: GlcNAc β1-3GalNAc-Ser/Thr; disialyl-T: [Sia α 2-3Galβ 1–3(Siaα2-6)GalNAc]-Ser/Thr; Fuc: fucose; Gal: galactose; GalNAc: N-acetylgalactosamine; Glc: glucose; GlcNAc: N-acetylglucosamine; Lac: lactose (Gal β1-4Glc) Man: mannose; Mel: melibiose; Mg: magnesium; Mn: manganese; Sia: Sialic acid; sialyl T: Sia α2-3Galβ1-3GalNAc- Ser/Thr; T: Galβ 1-3GalNAc-Serine(Ser)/Threonine(Thr); Tn: GalNAc-Ser/Thr; Zn: zinc.