TABLE 1.

Primers and probes for TaqMan qPCR.

Gene name	Alternate name	Category	Primer 1	Primer 2	Probe #
Aif1	IBA1	M0	atctgccgtccaaacttga	ctaggtgggtcttgggaacc	67
P2ry12	P2ry12	M0	gtgggcgtaccctacagaaa	aggcagccttgagtgttctt	109
Tgfb1	TGFβ	M0	tggagcaacatgtggaactc	gtcagcagccggttacca	72
Tmem119	Tmem119	M0	gtcactccatcccagtttcac	ggaccatgttgagctatggaa	110
Fcgr2b	CD32	M1	tactgtggacagccgtgcta	ttgaccacagcctttggaa	3
Ifng	IFNγ	M1	atctggaggaactggcaaaa	ttcaagacttcaaagagtctgagg	21
IL1b	IL1β	M1	agttgacggaccccaaaag	agctggatgctctcatcagg	38
Tnf	TNFα	M1	gccaacatccctacctctcc	ccccagggcaaaggtaat	92
Nos2	iNOS	M1	ctttgccacggacgagac	tcattgtactctgagggctgac	13
Arg1	Arg1	M2	ggcaaggtgatggaagagac	aggtgaatcggccttttctt	3
Chil3	Ym1	M2	ggtctgaaagacaagaacactgag	gagaccatggcactgaacg	88
IL10	IL10	M2	cagagccacatgctcctaga	tgtccagctggtcctttgtt	41
Trem2	Trem2	M2	cgagaggctgaggtcctg	tctccagcatcttggtcatcta	22
Tbp	TBP	HK	ggtcgcgtcattttctcc	gggttatcttcacacaccatga	107

Gene targets (plus common name), gene category with regard to microglial state, and primer pairs and TaqMan probes used to detect them in qPCR analysis.HK, housekeeping.