TABLE 3.
Expression of microglial transcripts in optic nerve.
| Target | Category | Method | Sham-Veh | Impact-Veh | Impact-Ral5 | Impact-Ral10 |
| IBA1 | M0 | qPCR | 100.0% | 181.7% | 141.7% | 194.2% |
| MERTK | M0 | NanoString | 100.0% | 89.3% | 91.7% | 112.4% |
| P2ry12 | M0 | qPCR/Nano | 100.0% | 124.4% | 93.5% | 133.7% |
| SPARC | M0 | NanoString | 100.0% | 134.8% | 130.5% | 138.6% |
| TGFβ | M0 | qPCR | 100.0% | 159.6% | 145.2% | 141.3% |
| Tmem119 | M0 | qPCR/Nano | 100.0% | 148.0% | 123.8% | 128.0% |
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| CD16 | M1 | NanoString | 100.0% | 157.1% | 203.2% | 192.6% |
| CD32 | M1 | qPCR/Nano | 100.0% | 228.4% | 215.9% | 306.9% |
| CD86 | M1 | NanoString | 100.0% | 170.6% | 119.1% | 214.2% |
| IFNγ | M1 | qPCR/Nano | 100.0% | 85.2% | 67.8% | 84.0% |
| IL1β | M1 | qPCR/Nano | 100.0% | 115.6% | 109.3% | 132.0% |
| IL6 | M1 | NanoString | 100.0% | 85.9% | 91.0% | 67.6% |
| IL12p40 | M1 | NanoString | 100.0% | 77.2% | 75.3% | 70.3% |
| iNOS | M1 | qPCR/Nano | 100.0% | 99.3% | 117.6% | 110.5% |
| MCP-1 | M1 | NanoString | 100.0% | 82.1% | 261.8% | 79.9% |
| Tlr2 | M1 | NanoString | 100.0% | 250.5% | 259.1% | 303.4% |
| TNFα | M1 | qPCR | 100.0% | 242.3% | 156.8% | 79.2% |
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| Arg-1 | M2 | qPCR/Nano | 100.0% | 72.8% | 54.2% | 146.6% |
| CD36 | M2 | NanoString | 100.0% | 114.2% | 64.5% | 157.4% |
| CD206 | M2 | NanoString | 100.0% | 122.0% | 130.6% | 195.7% |
| Fizz1 | M2 | NanoString | 100.0% | 62.7% | 81.6% | 72.4% |
| IL4rα | M2 | NanoString | 100.0% | 107.6% | 112.2% | 106.8% |
| IL10 | M2 | qPCR/Nano | 100.0% | 92.8% | 115.4% | 92.8% |
| IL13 | M2 | NanoString | 100.0% | 95.9% | 93.6% | 81.4% |
| Trem2 | M2 | qPCR/Nano | 100.0% | 146.8% | 158.7% | 173.6% |
| Ym1 | M2 | qPCR/Nano | 100.0% | 132.3% | 176.8% | 219.6% |
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| All | M0 | qPCR/Nano | 100.0% | 139.6% | 121.0% | 141.4% |
| All | M1 | qPCR/Nano | 100.0% | 144.9% | 152.4% | 149.1% |
| All | M2 | qPCR/Nano | 100.0% | 105.2% | 109.7% | 138.5% |
| All | M1/M2 | qPCR/Nano | 1.00 | 1.38 | 1.39 | 1.08 |
Expression was assessed 3 days after impact TBI. Expression levels for transcripts examined by both qPCR and NanoString were averaged. Data are expressed as a percentage compared to sham-vehicle mice.