TABLE 4.

Expression of microglial transcripts in retina.

Target	Category	Method	Sham-Veh	Impact-Veh	Impact-Ral5	Impact-Ral10
IBA1	M0	qPCR	100.0%	74.7%	51.2%	124.5%
MERTK	M0	NanoString	100.0%	127.8%	97.6%	80.9%
P2ry12	M0	qPCR/Nano	100.0%	292.4%	92.7%	154.0%
SPARC	M0	NanoString	100.0%	162.7%	117.2%	107.6%
TGFβ	M0	qPCR	100.0%	109.9%	77.8%	112.3%
Tmem119	M0	qPCR/Nano	100.0%	145.5%	89.6%	71.5%
CD16	M1	NanoString	100.0%	260.6%	105.9%	123.3%
CD32	M1	qPCR/Nano	100.0%	192.3%	93.3%	116.3%
CD86	M1	NanoString	100.0%	148.8%	145.1%	135.5%
IFNγ	M1	qPCR/Nano	100.0%	100.6%	105.8%	112.6%
IL1β	M1	qPCR/Nano	100.0%	190.7%	133.4%	216.5%
IL6	M1	NanoString	100.0%	68.9%	86.2%	81.3%
IL12p40	M1	NanoString	100.0%	128.7%	157.7%	146.3%
iNOS	M1	qPCR/Nano	100.0%	133.6%	102.3%	136.3%
MCP-1	M1	NanoString	100.0%	73.9%	90.8%	91.6%
Tlr2	M1	NanoString	100.0%	142.9%	130.0%	105.5%
TNFα	M1	qPCR	100.0%	44.6%	42.9%	128.3%
Arg-1	M2	qPCR/Nano	100.0%	54.7%	58.3%	53.8%
CD36	M2	NanoString	100.0%	82.3%	70.9%	59.5%
CD206	M2	NanoString	100.0%	171.8%	146.2%	119.7%
Fizz1	M2	NanoString	100.0%	184.6%	151.8%	166.3%
IL4rα	M2	NanoString	100.0%	90.2%	104.8%	83.9%
IL10	M2	qPCR/Nano	100.0%	114.6%	133.2%	127.7%
IL13	M2	NanoString	100.0%	126.1%	156.7%	149.3%
Trem2	M2	qPCR/Nano	100.0%	84.7%	95.3%	100.9%
Ym1	M2	qPCR/Nano	100.0%	126.5%	114.3%	184.8%
All	M0	qPCR/Nano	100.0%	152.2%	87.7%	108.5%
All	M1	qPCR/Nano	100.0%	135.1%	108.5%	126.7%
All	M2	qPCR/Nano	100.0%	115.1%	114.6%	116.2%
All	M1/M2	qPCR/Nano	1.00	1.17	0.95	1.09

Expression was assessed 3 days after impact TBI. Expression levels for transcripts examined by both qPCR and NanoString were averaged. Data are expressed as a percentage compared to sham-vehicle mice.