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. 2021 Oct 29;12:775506. doi: 10.3389/fphar.2021.775506

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Copyright © 2021 Xiang, Chen, Wang, Zhai, Sun, Feng, Duan, Zhang, Pan, Yan, Jin, Gao, Wen, Ma, Liu, Wang, Wu, Xie and Sui.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Erianin inhibited cell proliferation and triggered cell death in bladder cancer cells. (A) Cell proliferation of KU-19–19 and RT4 was assessed by CCK-8 assay after the treatment with different dose of erianin treatment for 24 h. (B) The cell morphology was observed by microscope. (C,D) Flow cytometry analysis of cell death by Annexin V-FITC/PI staining in KU-19–19 and RT4 cells were treated with erianin or DMSO control for 24 h, and the quantification of percentage of the cell death was shown. *p < 0.05,**p < 0.01. (E) The percentage of cells treated with erianin in each phase was assessed by flow cytometry.