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. 2021 Oct 29;12:775506. doi: 10.3389/fphar.2021.775506

FIGURE 3.

FIGURE 3

Erianin triggered ferroptosis in bladder cancer cells. (A,B) KU-19–19 and RT4 cells were treated with erianin for 24 h and ROS level was analyzed by a flow cytometer, the mean ± s.d. is shown. *p < 0.05. (C) The GSH level in KU-19–19 and RT4 cells was detected after the treatment with erianin for 24 h, *p < 0.05. (D) The MDA level in KU-19–19 and RT4 cells was detected after the treatment with erianin for 24 h, *p < 0.05,**p < 0.01. (E) The iron level in KU-19–19 and RT4 cells was detected after the treatment with erianin for 24 h, **p < 0.01. (F) KU-19–19 and RT4 cells were treated with erianin with or without the oxygen-derived free radicals scavenger GSH for 24 h, and cell viability was assayed, **p < 0.01, ***p < 0.001. (G) KU-19–19 and RT4 cells were treated with erianin with or without the ROS scavenger NAC for 24 h, and cell viability was assayed, **p < 0.01, ***p < 0.001. (H) Several ferroptosis related proteins were determined by western blotting.