Fig. 1.

Immunohistochemical analysis of demyelination and microglia/macrophage activation after cuprizone intoxication for 5 weeks. (a) Representative anti-PLP stained sections from a control and 5-week cuprizone intoxicated mouse. (b) Results of the densitometric evaluation of anti-PLP stained sections. (c) Representative LFB/PAS stained sections from a control and 5-week cuprizone intoxicated mouse. (d) Evaluation of the extent of demyelination of LFB/PAS stained sections. (e) Representative anti-IBA1 stained sections from a control and 5-week cuprizone intoxicated mouse. (f) Results of the densitometric evaluation of anti-IBA1 stained sections. In total, five to six animals per group were processed; two independent experiments were performed. Significant differences are indicated by asterisks (*p < 0.05, **p < 0.01, ***p < 0.001). Scale bar = 10 µm