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. 2021 Nov 11;11:22099. doi: 10.1038/s41598-021-01568-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fluorescence microscopy images of P.f.-RBCs after passing through the microfluidic devices (left column). Infected cells are shown as DAPI⁺ RESA⁺ (blue and green). White arrows indicate pitted cells (DAPI^-RESA⁺, green). Corresponding bright field images of the same fields are shown in the right column. The images were obtained with an epifluorescence microscope (Leica AF6000) and modified using ImageJ software (version 1.41n Wayne Rasband, NIH, (https://imagej.nih.gov/ij/).