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. 2021 Nov 11;12:6540. doi: 10.1038/s41467-021-26750-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Sagittal section of joints from CD4-CKO; Rosa26-mTmG mice were immunostained with marker protein GFP, transcriptional regulator SOX9 and nuclear counterstain DAPI. a, b Immunofluorescence staining images show SOX9 expression in all chondrocytes and GFP expression in hypertrophic chondrocytes in knee and spine section of P3 CD4-CKO; Rosa26-mTmG mice. a Blue dotted box shows cartilage mold, and green dotted box indicates hypertrophic zone of the growth plate. c, d Representative images of 1-month-old CD4-CKO; Rosa26-mTmG mice show GFP⁺ chondrocytes consisted in hypertrophic zone of femur and spine. e, f Immunofluorescence analysis of articular cartilage and growth plate (e) and chondroma (f) of the tibia in 7-month-old CD4-CKO; Rosa26-mTmG mice show GFP⁺ differentiated chondrocytes in the growth plate and articular cartilage of mature CD4-CKO;Rosa26-mTmG mice. e The area inside the two white dotted lines is articular cartilage (AC) (upper) and growth plate (GP) (under). a–f Scale bars, 100 μm. Data are representative of three independent experiments.