Figure 5.
Characterization of Zif-FLAP binding to CD25. (a) Specific binding of Zif-FLAP candidates to K562/CD25 cells. Binding of the five peptides to fixed K562/CD25 cells was detected using SA-AF488 (green). Scale bar = 50 μm. (b) Binding of Zif-FLAPs and daclizumab to CD25-Fc as monitored by BLI. Biotinylated Zif-FLAPs or daclizumab (dac) was immobilized on streptavidin sensors and exposed to various concentrations of CD25-Fc. Representative sensorgrams with corresponding KD ± SEM values calculated from three separate experiments are shown.