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. 2021 Oct 29;12:774323. doi: 10.3389/fimmu.2021.774323

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Copyright © 2021 Zhang, Xie, Mo, Zhang, Lin and Zhang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ACSL1 promoted inflammation in MDMs. (A–F) Caspase-1, caspase-3, and caspase-8 activity assay kits were used to analyze caspase-1 (A, B), caspase-3 (C, D), and caspase-8 (E, F) enzyme activities in ACSL1 overexpressed cells or ACSL1 knockdown cells followed by ALV-J infection for 3 and 6 h. (G–J) The supernatants were harvested to examine the levels of IL-1β (G, H) and IL-18 (I, J) by ELISA. (K, L) The supernatants were harvested to examine the concentration of NO by NO commercial kit. Data shown are the means ± SEM (n=3). P values were calculated using two-tailed unpaired Student’s t-test. Differences with P < 0.05 were considered significant. *P < 0.05, **P < 0.01.