Fig. 8. Nitrate inhibits symbiotic signalling via Nlp1 and Nlp4.

A A heatmap of NF signalling gene expression at different times following nitrate exposure. B–E Relative transcript abundance of Nfr1 (B), Nfr5 (C), Nsp2 (D), Ern1 (E) and Nin (F) by qRT-PCR 1 dpi with M. loti in the absence and presence of 5 mM KNO₃ in the indicated plant genotypes. Ubiquitin is used as a reference gene. Bars show mean ± SE for for the indicated n values. Significant differences between nitrate presence and absence conditions is annotated with P values as determined by Student’s t test. Gene IDs and expression values are shown in Supplementary Table 4.