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. 2021 Nov 11;12:6500. doi: 10.1038/s41467-021-26770-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Schematic experimental workflow for the study. A total of 34 freshly resected lung tumor specimens were collected from 25 patients, and normal lung tissues were collected from 18 patients as controls. Samples from patients 1 to 22 were processed using 10X Chromium V2 kits, while samples from patients 23 to 25 were used as the validation dataset and processed using 10X Chromium V3 kits. b UMAP visualization of 16 major cell types identified and color-coded by their associated clusters. c UMAP visualization of the 16 major cell types identified and color-coded by tumor or normal lung origin. d UMAP visualization of 16 major cell types identified and color-coded by histologic stages. LUAD: lung adenocarcinoma; UMAP: Uniform Manifold Approximation and Projection.