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. 2021 Nov 11;11:22119. doi: 10.1038/s41598-021-01652-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Multivariate data analysis of the influence of the cellular passage, processing batch and compound treatment by PCA and ASCA. Score plots from the analysis of 166 annotated metabolites based on cell passage (a), processing batch (b) and treatment (c). ASCA PC1 and PC2 scores plots for the factors ‘Passage’ (i.e. X_Passage) (d), ‘Batch' (i.e. X_Batch)’ (e), and ‘Treatment’ (i.e. X_Treatment) (f).