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. 2021 Nov 11;12:6504. doi: 10.1038/s41467-021-26764-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 9 — a Photomicrographs of duct-like structures generated from hPSC-derived cholangiocytes in the liver of TK-NOG mouse after 6 weeks of transplantation. HE and DAB staining with human specific antibodies. Scale bar represents 50 μm. b Confocal image of a histological section in the liver of TK-NOG mouse after 6 weeks of transplantation showing duct structures with primary cilia (green) counterstained with CK7 (red) and DAPI (blue). Scale bar represents 20 μm. c Confocal image of a histological section in the liver of TK-NOG mouse after 6 weeks of transplantation co-expressing human CK19 (red) and mouse CK19 (green). Scale bar represents 50 μm. d Confocal image of histological section showing duct structures in the kidney of NSG mouse showing human CK7 (red). Scale bar represents 100 μm. e Left: high magnification image of confocal microscopy demonstrating that hPSC-derived cholangiocyte display co-localization of CK7 (red) with acetylated α-tubulin (green) in the mouse kidney subcapsular space. Scale bar represents 20 μm. Right: high magnification image of confocal microscopy demonstrating that hPSC-derived cholangiocytes display co-localization of CK7 (red) with human mitochondria (green) in the mouse kidney subcapsular space. Scale bar represents 50 μm.