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. 2021 Nov 11;11:22126. doi: 10.1038/s41598-021-01469-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Optimization of enzyme balance by mixing two strains expressing P450/CPR and CDH enzymes. Strains expressing P450 and CPR (Mpa strain; BL21(DE3) pCDF-CYP71D18-ATR2) or CDH (Hc strain; BL21(DE3) pET-ISPD) were mixed in various ratios to determine the optimum balance for converting (−)-limonene to (−)-carvone. Varied P450/CDH input results in different (−)-carvone (3) production together with by-product dihydrocarvone (6) production.