Fig. 4.

(a) Flow cytometry analysis of apoptosis stage on B16 cells after different treatments. (b) The quantitative results of cell death percentage, including late apoptosis cells and dead cells. (c) The cell cycle distribution of B16 cells after different treatments. (d) The quantitative analysis of cell cycle distribution. (e) Western blot assays for protein expression in the B16 cells after different treatments and the grayscale analysis results of the protein bands, compared to the corresponding β-actin protein. (n = 3, *P < 0.05, **P < 0.01).