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(a) The TUNEL assay. (b) HE, Ki67 and HSP90 antigen immunohistochemistry-stained sections of B16 tumors, and (c–e) the corresponding positive expression calculated by the ImagePro Plus software (Mean ± SD, n = 3). (f) Western blot assays for protein expression in tumors after different treatments and (g) the grayscale analysis results of the protein bands, compared to the corresponding β-actin protein. (n = 3, *P < 0.05, **P < 0.01).
