Table 1.

Characterization of Vi-ΔVP8* conjugates.

Conjugates	ADH linkers per protein	Recovery (%)		Average size (kDa)	Vaccine Formulation
		Vi	ΔVP8*		Vi (μg)	ΔVP8* (μg)
Vi-ΔVP8*-P[4]	4	35	10	2665	5	1.0
Vi-ΔVP8*-P[6]	6	33	11	2583	5	1.1
Vi-ΔVP8*-P[8]	5	44	15	2773	5	1.5
Multivalent formulation	–	–	–	–	15	3.6

¹Recovery of each conjugate obtained was calculated on the basis of Vi concentration and protein concentration.

²Vi concentration was based on Hestrin assay.

³ΔVP8* protein concentration was based on Bradford assay.

⁴Multivalent formulation is the mixture of Vi-ΔVP8*-P[4], P[6], and P[8].