TABLE 1.
Yeast strains used in this study
| Strain | Genotype | Source or reference |
|---|---|---|
| A991 | MATa bar1Δ cdc20-1 | 75 |
| JC310 | MATa cdc28-13 3X (GAL-CDC5-3XMYC:: URA3) | 9 |
| JC311 | MATa cdc28-13 3X (GAL-CDC5-3XMYC:: URA3) cdh1Δ1::LEU2 | 9 |
| K3413 | MATa cln1,2,3Δ::LEU2 MET3-CLN2::TRP1 ade2-1 ade3-22 his3-11,15 ura3-1 | 2 |
| MaV103 | MATa SPAL10::URA3 leu2-3,112 trp1-901 his3Δ200 ade2-101 gal4Δ gal80Δ can1rcyh2rGAL1::HIS3@LYS2 GAL1::lacZ@URA3 | 74 |
| YJB10 | K3413 MATα | This study |
| YJB11 | K3413 MATa/α | This study |
| YJB14 | MATa bar1Δ | This study |
| YJB86 | MaV103 [CDH1-pAS2] | This study |
| YJB87 | MaV103 [CDC20-pAS2] | This study |
| YJB90 | YJB11 HSL-HA::URA3 | This study |
| YJB97 | MaV103 [CDC20-pAS2 MAD2-pACT] | This study |
| YJB101 | MaV103 [CDC20-pAS2 HSL1Δkinase-pACT] | This study |
| YJB102 | MaV103 [CDC20-pAS2 HSL1-pACTII] | This study |
| YJB103 | MaV103 [CDC20-pAS2 HSL1K110A-pACTII] | This study |
| YJB104 | MaV103 [CDC20-pAS2 SNF4-pACT] | This study |
| YJB105 | MaV103 [CDH1-pAS2 HSL1Δkinase-pACT] | This study |
| YJB106 | MaV103 [CDH1-pAS2 HSL1-pACTII] | This study |
| YJB107 | MaV103 [CDH1-pAS2 HSL1K110A-pACTII] | This study |
| YJB108 | MaV103 [CDH1-pAS2 SNF4-pACT] | This study |
| YJB115 | MATa bar1Δ cdc23-1::URA3 | This study |
| YJB121 | MATa bar1Δ hsl1Δ::TRP1 | This study |
| YJB123 | MATa bar1Δ GAL-HSL1-HA::TRP1 | This study |
| YJB124 | MATa bar1Δ cdc20-1 GAL-HSL1-HA::TRP1 | This study |
| YJB125 | MATa bar1Δ::URA3 cdc23-1 GAL-HSL1-HA::TRP1 | This study |
| YJB131 | MATa/α HSL1/hsl1Δ::TRP1 CDC23/cdc23-1 | This study |
| YJB133 | MATα hsl1Δ::TRP1 | This study |
| YJB156 | YJB123 [CDC20-pEG-KT] | This study |
| YJB157 | YJB123 [pEG-KT] | This study |
| YJB165 | MATa bar1Δ cdc28-13::URA3 | This study |
| YJB168 | MATa bar1Δ hsl1Δ::TRP1 HSL1-HA::LEU2 | This study |
| YJB172 | MATa/α HSL1/hsl1Δ::TRP1 CDC23/cdc23-1 SWE1/swe1Δ::LEU2 | This study |
| YJB177 | MATa/α HSL1/hsl1Δ::TRP1 CDC28/cdc28-13::URA3 | This study |
| YJB178 | MATa bar1Δ GAL-HSL1Δkinase-HA::LEU2 | This study |
| YJB184 | MATa bar1Δ GAL-HSL1K110A-HA::LEU2 | This study |
| YJB185 | MATa bar1Δ cdc20-1 GAL-HSL1Δkinase-HA::LEU2 | This study |
| YJB189 | MATa bar1Δ GAL-HSL1-HA: TRP1 swe1Δ::URA3 | This study |
| YJB190 | MATa bar1Δ GAL-HSL1K110A-HA::LEU2 swe1Δ::URA3 | This study |
| YJB191 | MATa bar1Δ GAL-HSL1Δkinase-HA::LEU2 swe1Δ::URA3 | This study |
| YJB197 | MATa/α HSL1/hsl1Δ::TRP1 CDC28/cdc28-13::URA3 SWE1/swe1Δ::LEU2 | This study |
| YJB198 | JC310 GAL-HSL1-HA::HIS3 | This study |
| YJB199 | JC311 GAL-HSL1-HA::HIS3 | This study |
| YJB218 | YJB123 [CDH1-pEG-KT] | This study |
| YJB221 | YJB14 [CDC20-pEG-KT] | This study |
| YJB222 | YJB14 [CDH1-pEG-KT] | This study |
| YJB223 | YJB178 [CDC20-pEG-KT] | This study |
| YJB224 | YJB178 [CDH1-pEG-KT] | This study |
| YJB225 | YJB184 [CDC20-pEG-KT] | This study |
| YJB226 | YJB184 [CDH1-pEG-KT] | This study |
| YJB229 | MATa bar1Δ GAL-HSL1db-mut-HA::LEU2 | This study |
| YJB230 | YJB229 [CDC20-pEG-KT] | This study |
| YJB231 | YJB229 [CDH1-pEG-KT] | This study |
| YJB247 | MATa bar1Δ GAL-GIN4-HA::LEU2 | This study |
| YJB248 | MATa bar1Δ GAL-KCC4-HA::LEU2 | This study |