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Yeast two-hybrid interactionsa
| AD fusion | β-Galactosidase activity (Miller units) for interaction with DB fusionb:
|
|
|---|---|---|
| Cdc20p | Cdh1p | |
| Hsl1pΔkinase | 66.4 | 21.0 |
| Hsl1p | 9.0 | 9.0 |
| HSL1pK110A | <0.1 | 4.0 |
| Mad2p | 59.5 | NDc |
| Snf4p | <0.1 | <0.1 |
β-Galactosidase activity for interactions between Cdc20p or Cdh1p DNA-binding domain (DB) fusions with the listed Gal4 activation domain (AD) fusion proteins.
Each numerical value represents the mean of three independent experiments.
ND, not determined.
