Figure 2.

Comparative abundance of the classical and alternative phenotype bearing splenic monocytes/macrophages/DCs at week 4 post-challenge. Representative FACS micrographs (A–C) and corresponding bar graphs (D–F) in the figure represent the percent frequency of Ly6C^high CAMs/CADCs and Ly6C^low AAMs/AADCs among (A, D) CD11b⁺F4/80^-SSC^lowmonocytes, (B, E) CD11b⁺F4/80⁺SSC^low macrophages, and (C, F) CD11b^-F4/80^-SSC^low DCs isolated from the spleen of mice belonging to various groups and profiled using flow cytometry. The groups included in the study and depicted in the FACS micrographs were: (a) Saline, (b) BCG, (c) rBCG30, (d) rBCG30+5,15-DPP (PVI), and (e) rBCG30+5,15-DPP (PII). The results depicted in the figure are representative of two independent experiments and are presented as means ± SEM of one of the two experiments with similar observations. The significance testing of differences between various groups was performed employing two-way ANOVA followed by Bonferroni’s multiple comparison post-test. The p values, <0.05(*), <0.01(**), <0.001(***) were considered as significant for analysis and interpretation of data. ns, non-significant.