Figure 7.

Memory T cell response at week 2 post-challenge. Antigen-specific CD4+ and CD8+ T central and effector memory response was assessed in the splenocytes isolated from various groups at 2 weeks PC. Shown in the figure are representative FACS plots depicting cumulative frequencies of (A) CD4+ and (B) CD8+ memory T cells from (a) Saline, (b) BCG, (c) rBCG30, (d) rBCG30+5,15-DPP (PVI), and (e) rBCG30+5,15-DPP (PII) groups, respectively. Bar graphs in the figure are depicting comparative magnitude (in percent) of effector memory (CD44^highCD62L^low) (Tem) and central memory (CD44^highCD62L^high) (Tcm) among (C) CD4+and (D) CD8+ T cells. The data were analyzed by employing Two-way ANOVA followed by Bonferroni’s multiple comparison test and are shown as means ( ± SEM) from one of the two independent experiments with similar observations performed in at least three biological replicates, where p<0.05(*), p<0.01(**), and p<0.001(***) were considered significant. ns, non-significant.