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. 2021 Oct 29;12:706727. doi: 10.3389/fimmu.2021.706727

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Copyright © 2021 Ahmad, Umar, Khan, Jamal, Gupta, Zubair, Gupta and Owais

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Evaluation of T cells memory response at 4 and 8 weeks post-challenge. Antigen-specific CD4+ and CD8+ T central and effector memory response was assessed in splenocytes isolated from various groups of mice at both 4 and 8 weeks PC. The bar graphs in the figure depict the comparative magnitude (in percent) of T effector (Tem) (CD44^highCD62L^low) and T central (Tcm) (CD44^highCD62L^high) cells among (A, C) CD4+, and (B, D) CD8+ memory T cells at 4 (upper panel) as well as 8 (lower panel) weeks PC, respectively. The data were analyzed by employing two-way ANOVA followed by Bonferroni’s multiple comparison test and are shown as means ( ± SEM) from one of the two independent experiments with similar observations performed in at least three biological replicates, where p<0.05(*), p<0.01(**), and p<0.001(***) were considered significant. ns, non-significant.