TABLE 2.

Composition of the ECM of planktonically- and biofilm-grown Mabs NJH12 in synthetic CF medium.

	ECM planktonic cells (μg/mg dry cells)	ECM biofilms (μg/mg dry cells)
Sugars	3.08 ± 0.27	5.85 ± 3.48
Proteins	13.07 ± 1.60	28.07 ± 2.08
Lipids	1.88 ± 0.45	36.96 ± 6.24
eDNA	0.11 ± 0.005	2.04 ± 0.21
[sugar:protein:lipid:eDNA] ratio	1:4.24:0.61:0.035	1:4.80:6.32:0.35

The ECM of 2 days-old planktonically grown cells and 5 days-old biofilm-grown Mabs NJH12 cells was collected and processed for DNA, lipid, protein, and sugar quantitative and qualitative analysis as described under section “Materials and Methods.” Bacterial cells recovered after their ECM was removed were freeze-dried and weighed. The amounts of ECM-derived DNA, lipids, proteins, and sugars provided below are expressed relative to the dry weight of bacilli stripped of their ECM. Shown are averages ± SD of triplicate cultures.