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Luciferase reporter assay done in HEK293T cells with pMIR-REPORT vector either containing the wild-type (wt, white) or a mutated 3′-UTR (gray) fragment of the indicated mRNA as a putative binding site for miR-181a. The control reporter was containing the binding site for a non-targeting control miR (NC). The gray dotted line indicates levels of binding for an empty pMIR-REPORT vector; n = 6; error bars correspond to mean values ± SD; **p ≤ 0.01 (Two-way ANOVA).
