Table 1.
HLA-G antibodies and their specificity and applications. Updated from Krijgsman D et al. (2020) (30) and Lin A et al. (2018) (31).
| HLA-G mAbs | Immunogen | Specificity | Applications |
|---|---|---|---|
| MEM-G/1 | Denatured bacterially expressed recombinant human HLA-G heavy chain | Denatured HLA-G heavy chain, all isoforms | IHC(F/P), WB |
| MEM-G/2 | Free heavy chain of all HLA-G isoforms | ||
| MEM-G/9 (IgG1) | Recombinant full length protein corresponding to human HLA-G | Native form of HLA-G1 and HLA-G5 isoform associated with β2M, Reactivity with HLA-G3 was reported | IHC(F), IP, FC, ELISA |
| MEM-G/11 | HLA-G1 | IHC(F), ICC, IP, FC, ELISA | |
| 01G | HLA-B27 transgenic mice immunized with H-2 identical murine cells transfected with HLA-G and human β2-microglobulin | Full-length HLA-G1 | |
| 87G (IgG2a) | HLA-G1 and HLA-G5, blocks interaction of HLA-G with inhibitory receptors | IHC(F), FC, ELISA, FUNC | |
| 2A12 | C-terminal amino acid sequence (22-mer) of soluble HLA-G5 and HLA-G6 proteins coupled to ovalbumin | HLA-G5 and HLA-G6 | IHC(F/P),FC, WB, ELISA |
| 5A6G7 (IgG1) | IHC(F/P), ICC, FC, WB, ELISA | ||
| 4H84 (IgG1) | Amino acids 61-83 of HLA-G α1 domain of human origin | An epitope in the HLA-G α1 domain | IHC(P), ICC, IP, WB, ELISA |
| G233 (IgG2a) | Murine L cells transfected with both human beta2-microglobulin and HLA-G | Several isoforms of HLA-G expressed in all populations of extravillous trophoblast. Without cross-react with any other MHC Class I antigens (HLA-A, -B, -C, -E, -F) | ICC/IF |
| MEM-G/4 | Recombinant human HLA-G denaturated heavy chain | denaturated HLA-G heavy chain | WB |
| 6D463 | IHC(F/P), IP, WB | ||
| 9-1F10 | Recombinant soluble HLA-G of human origin | HLA-G5 and HLA-G6 | IF, IP, WB, FC |