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. 2021 Oct 29;12:752940. doi: 10.3389/fphys.2021.752940

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Copyright © 2021 Cooper, Gloschat, Swift, Prudencio, McCullough, Jaimes and Posnack.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Simplified Workflow. (A) Representative raw image file from a Langendorff-perfused rat heart study (*.tif stack). Image properties (e.g., frame rate) are defined by the user. In our experimental setup, voltage and calcium signals can be analyzed from the same heart preparation. (B) Activation maps generated from raw image file demonstrate propagation away from the pacing electrode at the apex (left—voltage, right—calcium). (C) Generated action potential duration (left) and calcium transient duration (right) maps. (D) Representative traces from the raw image file.