TABLE 1.

Agrobacterium-mediated transformation efficiency using the binary vector pCEiEPSPS and pCP4-EPSPS.

Vector	Selective agent	Selection efficiency (%)	Regeneration efficiency (%)	Rooting efficiency (%)	Positive transgene events by PCR (%)	Final transformation efficiency (%)
pCEiEPSPS	3 mM glyphosate	55.18 ± 7.21a	92.61 ± 4.89a	82.87 ± 3.98a	100 ± 0a	42.07 ± 4.12a
	5 mM glyphosate	58.51 ± 14.37a	92.66 ± 2.25a	94.02 ± 4.24b	100 ± 0a	51.24 ± 14.16a
pCP4-EPSPS	3 mM glyphosate	60.63 ± 14.04a	81.87 ± 2.06b	93.21 ± 6.41b	100 ± 0a	45.386.59a
	5 mM glyphosate	59.33 ± 11.33a	82.85 ± 8.08b	93.92 ± 6.68b	100 ± 0a	46.31 ± 0.65a
pC1300	50 mg/L hygromycin	47.65 ± 2.73a	89.11 ± 3.47a	65.95 ± 15.68c	86.67 ± 2.36b	27.79 ± 6b

Data are the means ± SDs, different letters represent the significant difference by t-test, p < 0.05.