TABLE 2.
Transgene copy number of the binary vector pCEiEPSPS and pCP4-EPSPS detected by segregation and quantitative PCR (qPCR).
| Binary vector | Transgenic line | Total progeny | Resistant progeny | Susceptible progeny | Segregation ratio | X2c | P-value | Predicted copy number by segregation | Predicted copy number by qPCR |
| pCEiEPSPS | L1 | 36 | 8 | 28 | 3:1 | 50.70 | p < 0.05 | Complicated | 1 |
| L2 | 40 | 32 | 8 | 3:1 | 0.30 | p > 0.05 | 1 | 1 | |
| L3 | 32 | 24 | 8 | 3:1 | 0.04 | p > 0.05 | 1 | 3 | |
| L4 | 28 | 14 | 14 | 3:1 | 8.05 | p < 0.05 | Complicated | 2 | |
| L5 | 52 | 47 | 5 | 15:1 | 0.51 | p > 0.05 | 2 | 3 | |
| L6 | 36 | 34 | 2 | 15:1 | 0.03 | p > 0.05 | 2 | 2 | |
| L7 | 42 | 31 | 11 | 3:1 | 0.00 | p > 0.05 | 1 | 5 | |
| L8 | 44 | 32 | 12 | 3:1 | 0.03 | p > 0.05 | 1 | 1 | |
| L9 | 38 | 27 | 11 | 3:1 | 0.14 | p > 0.05 | 1 | 1 | |
| L10 | 25 | 14 | 11 | 3:1 | 3.85 | p < 0.05 | Complicated | 1 | |
| L11 | 18 | 18 | 0 | 15:1 | 0.37 | p > 0.05 | ≥2 | 1 | |
| L12 | 25 | 7 | 18 | 3:1 | 27.00 | p < 0.05 | Complicated | 2 | |
| L13 | 36 | 35 | 1 | 15:1 | 0.27 | p > 0.05 | ≥2 | 3 | |
| L14 | 30 | 24 | 6 | 3:1 | 0.18 | p > 0.05 | 1 | 1 | |
| L15 | 45 | 44 | 1 | 15:1 | 0.65 | p > 0.05 | ≥2 | 7 | |
| L16 | 48 | 36 | 12 | 3:1 | 0.03 | p > 0.05 | 1 | 2 | |
| Average copy number | 2.3 ± 1.64 | ||||||||
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| pCP4-EPSPS | L1 | 32 | 22 | 10 | 3:1 | 0.38 | p > 0.05 | 1 | 1 |
| L2 | 45 | 33 | 15 | 3:1 | 0.94 | p > 0.05 | 1 | 1 | |
| L5 | 40 | 30 | 10 | 3:1 | 0.03 | p > 0.05 | 1 | 1 | |
| L7 | 27 | 25 | 2 | 15:1 | 0.02 | p > 0.05 | 2 | 3 | |
| L8 | 36 | 36 | 0 | 15:1 | 1.45 | p > 0.05 | ≥2 | 3 | |
| L9 | 54 | 52 | 2 | 15:1 | 0.24 | p > 0.05 | ≥2 | 4 | |
| L10 | 20 | 2 | 18 | 3:1 | 41.67 | p < 0.05 | Complicated | 2 | |
| L11 | 45 | 42 | 3 | 15:1 | 0.04 | p > 0.05 | 2 | 2 | |
| L12 | 25 | 24 | 1 | 15:1 | 0.00 | p > 0.05 | ≥2 | 3 | |
| L13 | 26 | 21 | 5 | 3:1 | 0.21 | p > 0.05 | 1 | 2 | |
| L14 | 27 | 21 | 6 | 3:1 | 0.01 | p > 0.05 | 1 | 1 | |
| Average copy number | 2.1 ± 1.00 | ||||||||