TABLE 4.
Glyphosate resistance analysis of the T1 transgenic lines in the field.
| Binary vector | Transgenic line | Glyphosate dosage (g a.e./m2) | Plant height | No. of panicles | Panicle length | 1,000-grain weight | Grain No. per panicles | Filled grains per panicle | Seed-set rate (%) | Yield per plant |
| ZH11 | CK | 80.52 ± 4.73a | 5.67 ± 1.44a | 20.04 ± 1.77a | 23.8 | 95.49 ± 18.2a | 53.28 ± 18.1a | 55.18 ± 13.09a | 8.93 ± 3.33a | |
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| pCEiEPSPS | L2 | CK | 81.41 ± 5.45a | 6.17 ± 0.68a | 19.44 ± 1.79a | 23.8 | 90.77 ± 26.1a | 55.22 ± 20.2a | 66.06 ± 9.81a | 8.93 ± 2.25a |
| L8 | 83.60 ± 4.70a | 6.97 ± 1.83a | 18.50 ± 2.30a | 23.0 | 88.00 ± 25.4a | 48.66 ± 10.8a | 53.16 ± 13.00a | 7.93 ± 2.30a | ||
| L9 | 79.75 ± 4.01a | 7.33 ± 2.21a | 19.33 ± 2.03a | 22.5 | 99.16 ± 18.0a | 46.83 ± 17.5a | 46.44 ± 11.75a | 7.30 ± 1.02a | ||
| L14 | 80.75 ± 1.99a | 5.83 ± 2.03a | 20.35 ± 1.47a | 21.2 | 100.29 ± 21.8a | 61.28 ± 12.3a | 56.32 ± 8.97a | 7.85 ± 2.58a | ||
| pCP4-EPSPS | L1 | 84.00 ± 1.70a | 7.67 ± 1.24b | 17.37 ± 1.87c | 23.9 | 87.68 ± 28.1a | 52.74 ± 19.0a | 60.15 ± 9.08a | 6.32 ± 1.81a | |
| L2 | 82.16 ± 6.02a | 8.50 ± 1.89b | 20.05 ± 2.09a | 24.4 | 97.93 ± 22.1a | 66.11 ± 17.4a | 61.46 ± 10.59a | 9.95 ± 2.61a | ||
| L5 | 84.50 ± 2.91a | 6.33 ± 0.74a | 20.61 ± 1.47a | 23.7 | 104.68 ± 21.8a | 66.70 ± 15.1a | 63.78 ± 9.12a | 8.67 ± 2.17a | ||
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| pCEiEPSPS | L2 | 0.225 (2×) | 79.50 ± 2.30a | 8.50 ± 2.21b | 19.58 ± 1.73a | 24.7 | 92.10 ± 18.9a | 57.11 ± 15.3a | 62.32 ± 10.43a | 12.71 ± 2.03b |
| L8 | 87.25 ± 3.61b | 7.25 ± 1.29b | 20.35 ± 1.66a | 22.0 | 129.23 ± 26.2b | 80.50 ± 20.7b | 62.32 ± 11.25a | 10.63 ± 1.21a | ||
| L9 | 85.58 ± 4.43b | 9.00 ± 1.15b | 20.27 ± 1.09a | 24.5 | 119.57 ± 19.4b | 71.32 ± 15.1a | 59.96 ± 10.62a | 9.53 ± 2.03a | ||
| L14 | 86.75 ± 2.86b | 8.67 ± 2.28b | 20.92 ± 1.46a | 23.4 | 113.40 ± 25.7a | 68.24 ± 19.0a | 60.47 ± 11.76a | 10.31 ± 2.39a | ||
| pCP4-EPSPS | L1 | 78.25 ± 3.99a | 9.33 ± 1.97b | 18.45 ± 4.91a | 22.4 | 94.60 ± 24.6a | 58.28 ± 17.6a | 62.61 ± 11.33a | 10.55 ± 2.63a | |
| L2 | 81.75 ± 4.12a | 8.83 ± 3.48b | 20.55 ± 1.93a | 21.5 | 104.03 ± 23.6a | 70.08 ± 20.7a | 67.37 ± 11.06a | 10.78 ± 3.40a | ||
| L5 | 82.75 ± 2.59a | 6.00 ± 1.15a | 20.61 ± 2.04a | 24.2 | 98.40 ± 25.8a | 66.08 ± 19.8a | 66.47 ± 12.28a | 10.05 ± 2.09a | ||
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| pCEiEPSPS | L2 | 0.56 ± 25 (5×) | 76.41 ± 1.94a | 7.83 ± 2.33b | 18.91 ± 1.66a | 24.3 | 95.94 ± 19.5a | 59.40 ± 10.3a | 60.24 ± 7.27a | 6.62 ± 1.89a |
| L8 | 86.50 ± 4.61b | 6.50 ± 1.70a | 19.48 ± 1.59a | 22.8 | 101.32 ± 41.7a | 70.46 ± 19.9a | 62.70 ± 9.95a | 7.80 ± 4.00a | ||
| L9 | 76.91 ± 4.01a | 6.67 ± 1.69a | 19.54 ± 0.91a | 21.9 | 109.67 ± 15.3a | 65.72 ± 17.6a | 59.24 ± 10.50a | 6.20 ± 1.96a | ||
| L14 | 71.81 ± 3.70c | 6.30 ± 0.81a | 20.74 ± 1.44a | 22.5 | 102.20 ± 19.5a | 61.84 ± 16.4a | 60.50 ± 11.14a | 5.65 ± 2.34a | ||
| pCP4-EPSPS | L1 | 72.75 ± 2.62c | 7.67 ± 2.28b | 17.77 ± 1.17c | 21.5 | 91.02 ± 17.5a | 56.15 ± 11.6a | 62.54 ± 11.14a | 6.82 ± 2.19a | |
| L2 | 77.66 ± 2.19a | 8.33 ± 2.05b | 18.68 ± 1.02a | 21.9 | 100.35 ± 21.7a | 55.85 ± 15.9a | 55.61 ± 10.84a | 8.08 ± 1.58a | ||
| L5 | 78.91 ± 5.71a | 5.83 ± 0.68a | 20.52 ± 0.84a | 24.4 | 94.71 ± 15.6a | 60.46 ± 10.9a | 64.53 ± 10.27a | 8.77 ± 1.70a | ||
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| pCEiEPSPS | L2 | 1.125 (10×) | 76.41 ± 1.94a | 9.17 ± 1.34b | 19.44 ± 1.71a | 24.4 | 104.26 ± 17.2a | 61.94 ± 16.9a | 62.75 ± 17.43a | 9.05 ± 3.14a |
| L8 | 87.25 ± 3.35b | 9.50 ± 3.59b | 20.34 ± 2.36a | 20.6 | 123.00 ± 33.1b | 72.11 ± 24.5a | 58.78 ± 13.13a | 8.58 ± 2.90a | ||
| L9 | 82.16 ± 4.81a | 7.20 ± 1.32a | 21.18 ± 1.48a | 22.0 | 112.09 ± 15.6a | 71.21 ± 12.5a | 64.45 ± 12.83a | 7.08 ± 3.21a | ||
| L14 | 83.50 ± 3.02a | 7.33 ± 0.74b | 21.09 ± 1.46a | 20.8 | 115.50 ± 27.6a | 65.53 ± 14.7a | 58.61 ± 13.58a | 8.08 ± 2.69a | ||
| pCP4-EPSPS | L1 | 79.66 ± 1.81a | 7.00 ± 1.15a | 18.82 ± 1.48a | 20.7 | 109.22 ± 18.5a | 65.15 ± 17.1a | 60.40 ± 13.86a | 7.93 ± 2.99a | |
| L2 | 82.66 ± 4.00a | 9.50 ± 2.29b | 19.32 ± 1.41a | 22.5 | 117.13 ± 23.1a | 64.00 ± 20.3a | 57.37 ± 10.47a | 9.75 ± 3.57a | ||
| L5 | 84.16 ± 3.19a | 7.00 ± 1.63a | 20.87 ± 2.08a | 22.4 | 113.97 ± 32.0a | 69.43 ± 26.1a | 61.07 ± 11.59a | 8.55 ± 3.89a | ||
Data are means ± SDs, different letters represent the significant difference by t-test, p < 0.05.