Fig. 2.
Hydrogen induces tumor cell apoptosis at certain critical concentrations. (a) A series of morphology images of HCT116 cells from a live cell imaging microscope. (b) Schematic illustration of H2-emitting device. (c) The cell viability of HCT116 cells with different treatments. (d) The cell cycle of the HCT116 cells in the Ctrl and M − H2 groups; and (e) flow cytometry of cell apoptosis after different treatments and the corresponding apoptosis rate was statistically quantified. L-H2 and M − H2 groups represent the low and medium concentrations of H2 in the culture medium, respectively. The ctrl group represents no H2-treatment.