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. 2021 Aug 9;9:385–396. doi: 10.1016/j.bioactmat.2021.07.026

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© 2021 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 4 — The up-regulated P53 activates the mitochondrial apoptosis pathway by hydrogen. (a) The P53, cathepsins, Bax, Bcl-2 and LC3B expression after M − H₂, L-H₂ and Ctrl treatment, respectively. (b) Confocal fluorescence images of mitochondria membrane potential and apoptosis of HCT116 with or without H₂ treatment. The mitochondria membrane potential was stained by Mito-Tracker Red CMXRos (red), apoptosis cells were stained by Annexin V-FITC (green) and the nuclei were stained by Hoechst 33342 (blue). The scale bar is 25 μm. (c) The analysis of cytochrome C expression in HCT116 cells using the APC-conjugated cytochrome C antibody. Scale bar: 25 μm. (d) The analysis of the caspase-3 expression in HCT116 cells using FITC-conjugated cleaved caspase-3 antibody. Scale bar: 25 μm.