Figure 3.

Se restricts F. tularensis LVS replication in macrophages. BMDMs from Se-deficient, -adequate, or -supplemented mice were maintained ex vivo under Se deficient (0 nM), adequate (50 nM) or supplemented (200 nM) conditions (n=3/one biological replicate, n=3 biological replicates/diet group), infected with F. t LVS at an MOI of 1:100. (A) Intracellular growth of bacteria was enumerated at 24 hr post-infection. (B) Bacterial growth over 24 hr represented as fold change in bacteria enumerated at 2 hr post infection. Data are depicted as the mean of three biological replicates and error bars denote +SD. Statistical significance was assessed by One-way ANOVA with Tukey’s Multiple Comparison Test (*p< 0.05) and data are representative of three independent experiments. **p<0.01, ***p<0.001.