Figure 7.

Selenoproteins are differentially regulated between infected and uninfected macrophages. BMDMs from WT mice (n=3/group) were infected with F. tularensis LVS at an MOI of 1:10. Cells were harvested at 10hr p.i., and processed for RNA isolation and subsequent sequencing. Volcano plot of differentially expressed genes shows that 20 of 24 murine selenoproteins are differentially expressed when comparing infected BMDMs to uninfected controls, with six selenoproteins showing significance. Differential expression analysis was conducted using edgeR v. 3.30.0 (FDR <0.05).