FIGURE 1.

IC₅₀ values of temozolomide (TMZ), lomustine (CCNU), and nimustine (ACNU) for glioblastoma (GBM) cells. A, The indicated GBM cells were treated with different concentrations of TMZ, CCNU. or ACNU as indicated. After 72 h, the relative ratios of viable cell numbers of the treated cells in each condition were determined by WST‐8 assay, as described in the Materials and Methods, and plotted graphically. Data for U87MG (U87), U251MG (U251), and U 343MG (U343) cells are shown as blue lines and for U87‐R, U251‐R and U343‐R as red lines. ^* P <.05, ^** P <.01. B, The patient‐derived GBM cell line GS‐Y03 cells were treated with different concentrations of TMZ, CCNU, or ACNU, as indicated. After 72 h, the relative ratios of viable cell numbers in each condition were determined and plotted, as shown in (A)