FIGURE 3.

Effects of temozolomide (TMZ), lomustine (CCNU), and nimustine (ACNU) in inducing apoptosis in glioblastoma (GBM) cells as evaluated by immunoblotting. A, The indicated GBM cells were treated with 50 μM TMZ, CCNU, or ACNU. After 72 h, the whole cell lysates were obtained and analyzed by immunoblotting using the indicated primary antibodies. The antibody against GAPDH was used to confirm the amount of protein in each sample. B, The indicated GBM cells were treated with 200 μM TMZ or ACNU. After 72 h, the whole cell lysates were analyzed as in (A) C, U251 cells were treated with CCNU or ACNU in the presence or absence of pan‐caspase inhibitor Z‐Val‐Ala‐Asp(OMe)‐CH₂F (zVAD). After 72 h, the cell mortality rate was estimated as described in the Materials and Methods. ^* P <.05, ^** P <.01