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Imiquimod up‐regulated the expression of OX40 in the tumor microenvironment. (A) The correlation of TLR7 or TLR responsive elements and OX40 in liver hepatocellular carcinoma (LIHC) was computed through an online tool (http://gepia2.cancer‐pku.cn/#correlation). (B) When intratumoral injection either with normal saline (NS) or imiquimod once, tumors were excised 48 h later and OX40 expression of the CD3+CD4+ T cell subset analyzed by flow cytometry (n = 3). P‐value: ***, P < .0001
