FIGURE 4.

Dual inhibition of WEE1 and ATR further activates the STING signaling pathway in cancer cells. (A) Representative images of p‐TBK1 and p‐IRF3 positive cells in OVCAR8 cells (B) Quantification of positive cells in (A). (C, D) Western blotting of indicated proteins in OVCAR8 and ID8 cells. (E, F) Tissue sections were immunofluorescent stained with p‐TBK1 and p‐IRF3. (G) Quantification and statistical analysis of (E) and (F). (H) After silencing STING, let it stand for 72 h with or without a combination treatment. Western blot of specified proteins in OVCAR8 cells. (I) After exposure to drugs with or without H‐151 (500 nM). Representative image of the clonogenic assay (left) and quantitative analysis (right) in ID8 and OVCAR8 cells. *P < .05, **P < .01, ***P < .001, ****P < .0001