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. 2021 Oct 29;12:741219. doi: 10.3389/fphar.2021.741219

FIGURE 2.

FIGURE 2

SC75741 increases clearance of ALS-related pathogenic mutations-induced aggregation. (A). SH-SY5Y cells were transfected with indicated plasmids for 24 h, treated with or without 5 μM SC75741 for another 24 h, the GFP fluorescence was done using confocal microscopy. Scale bar, 20 μm. (B). SH-SY5Y cells were transfected with indicated plasmids for 24 h, treated with or without 5 μM SC75741 for another 24 h, the GFP fluorescence was analyzed by fluorescence microscopy. (data represent mean ± SD; n = 6, **** p < 0.0001, ns, not significant, two-tailed t test). (C). SH-SY5Y cells were transfected with indicated plasmids for 24 h, treated with or without 5 μM SC75741 for another 24 h. Cell lysates were immunoblotted with indicated antibodies. The numbers under the blot represent the gray scale quantification (GFP/Tubulin). (D). SH-SY5Y cells were transfected with GFP-SOD1 G93A for 24 h, treated with or without 5 μM SC75741 for another 24 h, the GFP fluorescence was done using confocal microscopy. Scale bar, 20 μm. (E). SH-SY5Y cells were transfected with GFP-SOD1 G93A for 24 h, treated with or without 5 μM SC75741 for another 24 h, the GFP fluorescence was analyzed by fluorescence microscopy. (data represent mean ± SD; n = 8, **** p < 0.0001, ns, not significant, two-tailed t test). (F). SH-SY5Y cells were transfected with GFP-SOD1 G93A for 24 h, treated with or without 5 μM SC75741 for another 24 h. Cell lysates were immunoblotted with indicated antibodies. The numbers under the blot represent the gray scale quantification (GFP/Tubulin).