Fig. 4.

Uptake of RBCEVs by endothelial cells at different blood flow velocities. (a) Images were taken with a laser confocal microscope. RBCEVs were injected into the heart. (b) Model diagram of zebrafish. (c) Blood flow velocities in the four different blood flow regions. (d) In the laser confocal image, the red puncta in the square represent RBCEVs at four speeds. (e) Number of RBCEVs uptake per visual field (scale bar = 500 μm). (f) In the laser confocal image, white arrows point to RBCEVs that were uptake by vascular endothelial cells at 3 h. (g) XYZ axis showed uptake in different directions. (h) Imaris 3D rendering and (i) front and lateral view (n = 8) (scale bar = 100 μm). Significance is indicated as P < 0.05 (*), or P < 0.001 (***).