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Physicochemical characterization of the NPs developed in this study. a) Size, PdI, ζ-potential and b) size distribution by intensity of bare and insulin-loaded UnTHCPSi NPs, UnTHCPSi@LNPs and UnTHCPSi@LNPs-Fc. Results are expressed as mean ± s.d. of three independent measurements of the same batch ( 3). c) ATR−FTIR spectra of the NPs at different steps of preparation.
