Fig. 4.

pH-Responsive behavior of the NPs. UnTHCPSi@LNPs, UnTHCPSi@LNPs-Fc and insulin-loaded UnTHCPSi@LNPs-Fc after incubation in different buffer solutions (pH 1.2, 5.5, 6.0, 6.5 and 7.4) for 2, 15 and 30 min. a) Size and PdI; results are expressed as mean ± s.d. of three independent measurements of the same batch ($n\ge$ 3); b) TEM images of the NPs (scale bars represent 200 nm).