Fig. 7.

Cytocompatibility of the developed NPs. Cell viability values (%) of Caco-2, HT29-MTX and Caco-2/HT29-MTX (ratio 90:10) when exposed to UnTHCPSi, UnTHCPSi@LNPs, and UnTHCPSi@LNPs-Fc assessed by CellTiter-Glo® luminescence assay. The ATP content was investigated after 6 and 24 h of incubation with the different NPs at different concentrations (25–400 μg). Cells were incubated with cell culture media, as negative control. The incubations were conducted at 37 °C and 5% CO₂. Two-way ANOVA was used for the statistical analyses of the measured values, followed by the Bonferroni post hoc test. Each data set was compared to the negative control. The level of significant differences was set at probabilities of *$p<$ 0.05, **$p<$ 0.01, ***$p<$ 0.001, and ****$p<$ 0.0001. Results are expressed as mean ± s.d. of four independent measurements ($n$ = 4).