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. 2021 Jul 27;9:411–427. doi: 10.1016/j.bioactmat.2021.07.028

Fig. 2.

Fig. 2

The proliferation of SMSCs were promoted by exposure to tFNAs. a. CCK-8 results of SMSCs proliferation under the treatment of tFNAs with different concentrations. Data are presented as mean ± SD (n = 4). b. Immunofluorescence micrographs of EdU staining (EdU: orange, nucleus: blue). Scale bars are 100 μm. c. Semi-quantitative analysis of EdU positive cell ratio based on images. Data are presented as mean ± SD (n = 3). d. Flow-cytometry results of the cell cycle of SMSCs treated with or without 250 nM tFNAs for 24 h. Data are presented as the mean ± SD (n = 3). Statistical analysis: *p < 0.05, **p < 0.01, ***p < 0.001, n.s. represents no significant difference.