Figure 3.

BAT BCAA catabolic defects directly contribute to obesity in DIO mice

(A) Representative HE and UCP1 staining of BAT. Scale bar, 200 μm.

(B) Rectal core body temperature following cold exposure at 8°C. n = 12 per group.

(C) Change in BAT temperature following treatment with noradrenaline. n = 6 per group.

(D) Body weight changes in the indicated groups. Resection of interscapular BAT or sham operation was performed on 12-week-old HFD-fed mice (HFD from 6 weeks) that were randomly divided into four groups. i.e., sham + vehicle (n = 6), sham + BT2 (n = 6), iBATx + vehicle (n = 12), and iBATx + BT2 (n = 12).

(E) 1-g oral glucose-tolerance test. n = 6 per group.

(F) Weights of the iWAT and eWAT in the indicated groups.

(G) Experimental design. (H) Body weight changes in the indicated groups. n = 12 per group.

(I) Rectal core body temperature following cold exposure at 8°C at 12 weeks of age. n = 8 per group. Data are shown as mean ± SEM (A–F, H, I); two-tailed unpaired Student's t test (A, F); two-way repeated measures ANOVA (E, H) followed by post-hoc unpaired t test (I). ∗∗p < 0.01, ∗∗∗p < 0.001. BAT, brown adipose tissue; BT2, 3,6-dichlorobenzo[b]thiophene-2-carboxylic acid; iBATx, interscapular BATectomy; KIC, ketoisocaproic acid; KIV, ketoisovaleric acid; KMV, keto-beta-methylvaleric acid; NS, not significant; UCP1, uncoupling protein 1; WAT, white adipose tissue.