Fig. 6.

Increased trabecular mineralization in Runx2 mutant mice. (A) Paraffin sections of the tibia from 3-days old littermates were stained with Von Kossa followed by alcian blue. Representative images of the tibia taken at 4x magnification are shown. Scale bar: 500 μm. (B) Representative 3D-image of trabecular bone from the femur of 3-days old Runx2^+/+ and Runx2^HC/HC littermates. Scale bar: 100 μm. (C) Trabecular bone parameters were analyzed from a 2.4 mm region immediately beneath the growth plate. Quantified data of bone parameters from Runx2^+/+ and Runx2^HC/HC littermates (n = 4) is presented in a box and whisker plot. BV; bone volume, TV; total volume, Tb.N; trabecular number, Tb.Th; trabecular thickness, Tb.Sp; trabecular space. (*P < 0.05, **P < 0.01, ***P < 0.001). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)