Figure 2.
Transcription and translation kinetics of B. subtilis following fusidic acid (FA) treatment and nonsense mutation
Cells were grown in gly + cAA medium.
(A) THE growth rates of B. subtilis in gly + cAA medium supplemented with/without 0.2 μg/mL FA. Data are represented as mean ± SD.
(B) The induction curve of the complete lacZ mRNA (green triangles) and LacZ protein (purple circles) for B. subtilis grown in gly + cAA medium supplemented with 0.2 μg/mL FA.
(C) Summary of the transcription and translation elongation rates of lacZ mRNA for B. subtilis0020 under normal condition, FA treatment, and nonsense mutation. Data are represented as mean ± SD.
(D) The induction kinetics of the lacZ mRNA for B. subtilis under FA treatment.
(E) The induction kinetics of the araAB mRNA for B. subtilis under FA treatment.
(F) The induction kinetics of a nonsense-mutated lacZ mRNA. The coding sequence of the 154th amino acid residue of the native lacZ, “TGG,” was mutated to “TAA” stop codon.
(G) The transcription processivity of lacZ mRNA in B. subtilis under normal condition, FA treatment, and nonsense mutation. The same as Figure 1G, the relative accumulation rates of mRNA sub-regions (slope of the linear induction curve) are plotted against the hybridization locations of primers for lacZ mRNA. (H) The transcription processivity of lacZ mRNA in E. coli under normal condition, FA treatment, and nonsense mutation. Data originate from Zhu et al (2019).