Figure 2.

Analysis of EAG response kinetics to pulsed bombykol stimuli in BmPBP1-knockout male moths

(A) Representative EAG responses to pulsed bombykol trains in BmPBP1-knockout (blue) and wild-type (red) male antennae at 0.17 Hz and 2 Hz. The stimulus was applied for 200 ms, as indicated by the solid line on the trace. The broken line indicates baseline level of each EAG trace.

(B) Parameters considered in the antennal response kinetics analysis of EAG recording to pulsed bombykol stimuli.

(C) Termination time of EAG responses to pulsed bombykol trains in BmPBP1-knockout (blue) and wild-type (red) antennae at 0.17 Hz (KO; n = 9, WT; n = 9) and 2 Hz (KO; n = 12, WT; n = 6). Error bars represent ±SEM. The asterisks indicate significant differences between the groups (∗∗p < 0.01, Student’s t test for paired samples).

(D) Valley amplitude of male antennae to bombykol stimuli in BmPBP1-knockout (blue) and wild type (red) at 0.17 Hz (KO; n = 9, WT; n = 9) and 2 Hz (KO; n = 16, WT; n = 7); 10,000 ng bombykol was used in BmPBP1-knockout and 100 ng of bombykol was used in wild-type males. Error bars represent ±SEM. The asterisks indicate significant differences between the groups (∗∗p < 0.01, Student’s t test for paired samples). NS indicates no significant difference.

See also Figure S1.