Figure 3.

Analysis of antennal temporal resolution in single-cell spike responses to pheromone pulse trains in BmPBP1-knockout male moths

(A) Comparison of spontaneous and elicited spike numbers. Spontaneous spikes were calculated with a time bin of 2 s before the first stimulus was presented to BmPBP1-knockout (blue; n = 9 at 0.17 Hz, n = 8 at 2 Hz) and wild-type (red; n = 13 at 0.17 Hz, n = 11 at 2 Hz) male antennae; spike numbers were calculated with a time bin of 5 s from the stimulus at 0.17 Hz and (B) with a time bin of 3.5 s after the stimulus at 2 Hz. Error bars represent ±SEM. NS indicates no significant difference according to Student’s t test for paired samples.

(C) Comparison of the termination time of spikes between BmPBP1-knockout and wild-type at 0.17 Hz. Error bars represent ±SEM. The asterisks indicate significant differences between the groups (∗∗p < 0.01, Student’s t test for paired samples).

(D) Representative spike responses of long sensilla trichodea in BmPBP1-knockout (blue) and wild-type (red) male antennae to pheromone pulse stimuli at 0.17 and 2 Hz. Solid lines on the trace represent the timing of intermittent bombykol stimuli.

(E and F) (E) Spike rate in time bins of 100 ms in BmPBP1-knockout (blue; n = 9) and wild-type (red; n = 13) male antennae at 0.17 Hz and (F) those of 100 ms in BmPBP1-knockout (blue; n = 8) and wild-type (red; n = 11) male antennae at 2 Hz. Error bars represent ±SEM. 10,000 ng bombykol is used as stimuli for BmPBP1-knockout male antennae and 1,000 ng bombykol is used as stimuli for wild-type male antennae.